par Dehaye, Jean-Paul 
;Marino, Aida;Soukias, Yves ;Poloczek, Piotr ;Winand, Jacques ;Christophe, Jean
Référence European journal of pharmacology, 151, 3, page (427-434)
Publication Publié, 1988-07
;Marino, Aida;Soukias, Yves ;Poloczek, Piotr ;Winand, Jacques ;Christophe, Jean Référence European journal of pharmacology, 151, 3, page (427-434)
Publication Publié, 1988-07
Article révisé par les pairs
| Résumé : | The muscarinic agonist, carbamylcholine, stimulated amylase secretion in rat parotid acini 6-fold, the 86Rb efflux 5-fold, the 45Ca efflux 5-fold and the accumulation of inositol monophosphate, bisphosphate, triphosphate and tetrakisphosphate 4-, 4-, 3- and 3-fold, respectively. The EC50 of carbamylcholine on these parameters were 0.4, 0.5, 1.3, 12, 12, 6 and 9 μM, suggesting spareness between phospholipase C activation and amylase secretion. These muscarinic responses were inhibited by four muscarinic antagonists with an order of potency on all parameters and on receptor occupancy (using N-[methyl-3H]scopolamine as a tracer): atropine>hexahydrosiladifenidol>pirnzepine>AF-DX 116. The pA2 of these antagonists on carbamylcholine-stimulated amylase secretion were 9.72 for atropine, 8.14 for hexahydrosiladifenidol, 7.16 for pirenzepine and 6.22 for AF-DX 116, indicating that the parotid muscarinic receptors were of an M2 subtype 83-fold more sensitive to hexahydrosiladifenidol than to AF-DX 116. © 1988. |
