par Alexandre, Sylvie 
;Paindavoine, Pascale ;Tebabi, Patricia ;Pays Deschutter, Annette ;Halleux, S;Steinert, Maurice ;Pays, Etienne
Référence Molecular and biochemical parasitology, 43, 2, page (279-288)
Publication Publié, 1990-12
;Paindavoine, Pascale ;Tebabi, Patricia ;Pays Deschutter, Annette ;Halleux, S;Steinert, Maurice ;Pays, Etienne Référence Molecular and biochemical parasitology, 43, 2, page (279-288)
Publication Publié, 1990-12
Article révisé par les pairs
| Résumé : | The expression site for the variant surface glycoprotein (VSG) gene of Trypanosoma brucei contains several genes of unknown function (ESAGs, for expression site-associated genes). Among these, ESAG 4 shows homology to eukaryotic adenylate/guanylate cyclase genes, in the region encoding the presumptive enzyme catalytic domain. This gene belongs to a family of related sequences, and hybridizes to the genomic DNA of other trypanosomatids, such as Trypanosoma congolense, Trypanosoma vivax and Trypanosoma mega. While ESAG 4 is transcribed only in bloodstream forms by a RNA polymerase resistant to alpha-amanitin, at least three other members of this family are transcribed in both bloodstream and procyclic forms, by a RNA polymerase sensitive to the drug. These genes encode different putative transmembrane proteins showing high sequence conservation in the region corresponding to the adenylate/guanylate cyclase catalytic domain. |
