par Lonez, Caroline 
;Legat, Amandine ;Vandenbranden, Michel ;Ruysschaert, Jean Marie
Référence Cellular and molecular life sciences, 65, 4, page (620-630)
Publication Publié, 2008-02
;Legat, Amandine ;Vandenbranden, Michel ;Ruysschaert, Jean Marie Référence Cellular and molecular life sciences, 65, 4, page (620-630)
Publication Publié, 2008-02
Article révisé par les pairs
| Résumé : | The inflammatory effect of unmethylated CpG DNA sequences represents a major obstacle to the use of cationic lipids for in vivo gene therapy. Although the mechanism of CpG-induced inflammatory response is rather well understood nowadays, few solutions have been designed to circumvent this effect in gene therapy experiments. Our previous work has shown that a refractory state towards inflammation can be elicited by preinjecting cationic liposomes. Here, we present evidence that diC14-amidine liposomes confer new anti-inflammatory properties to phospholipids from low-density lipoprotein (LDL) and even to synthetic phospholipids for which such an observation has not been reported so far. Whereas oxidation of LDL lipids was a prerequisite for any anti-inflammatory activity, lipid oxidation is no longer required in our experiments, suggesting that cationic lipids transport phospholipids through a different route and affect different pathways. This opens up new possibilities for manipulating inflammatory responses in gene therapy protocols but also in a general manner in immunological experiments. |
