par Pötter, E;Horne, Roland ;Scheumann, G F;Dralle, H;Costagliola, Sabine ;Ludgate, Marian;Vassart, Gilbert ;Dumont, Jacques ;Brabant, G
Référence Biochemical and biophysical research communications, 205, 1, page (361-367)
Publication Publié, 1994-11
Référence Biochemical and biophysical research communications, 205, 1, page (361-367)
Publication Publié, 1994-11
Article révisé par les pairs
Résumé : | Expression of thyrotropin receptor (TSHr) was analyzed in normal human thyroids and differentiated thyroid tumours by Northern blotting of total RNA and by Western blotting of detergent-solubilized membrane proteins with an antibody specific for the extracellular domain of TSHr. Under nonreducing conditions TSHr stained as a 90-kDa protein in normal thyroid tissue. Expression varied in differentiated carcinomas from normal to not detectable in parallel to steady state TSHr mRNA levels and TSH binding in a TSH binding assay. The negative expression of TSHr in some patients with differentiated carcinomas may have consequences for the TSH suppressive treatments of these patients. |