par Ghanem, Ghanem Elias 
;Legros, Franz;Lejeune, Ferdinand ;Fruhling, Janos
Référence Journal of immunological methods, 54, 2, page (223-232)
Publication Publié, 1982-10
;Legros, Franz;Lejeune, Ferdinand ;Fruhling, Janos Référence Journal of immunological methods, 54, 2, page (223-232)
Publication Publié, 1982-10
Article révisé par les pairs
| Résumé : | We have studied the behaviour of 125I-labelled alpha-MSH under different experimental conditions. Until now, the chloramine T method had been used by most investigators with variable results. We have tested three other labelling techniques based on 125I mild oxidation: (1) an enzymatic method with lactoperoxidase, (2) a sparingly soluble chloramine method (T.D.G.U.) and (3) modified chloramine T procedure, 'the iodine volatilization method'. Labelled hormone obtained after each kind of iodination was assayed for immunoreactivity. In addition, time course degradation was measured by classical RIA incubation procedures. Charcoal-dextran was used to separate bound and free antigen. We have found chloramine T-iodinated alpha-MSH to be significantly more damaged than preparations obtained by other methods and to be less stable when stored at -18 degrees C. No differences were found between the differently labelled 125I-labelled alpha-MSH fresh preparations in binding to surface receptors of human melanoma cell lines in culture. |
