par Perez-Careta, J;Avina-Cervantes, Gabriel;Debeir, Olivier ;Sanchez-Mondragon, J;May-Arrioja, Daniel May;Torres-Cisneros, M
Editeur scientifique Müller-Karger, Carmen;Wong, Sara;Cruz, Alexandra
Référence (September 24th-28th, 2007: Margarita Island, Venezuela), IV Latin American Congress on Biomedical Engineering 2007, Bioengineering Solutions for Latin America Health, Springer, Berlin, Heidelberg, page (1148-1151)
Publication Publié, 2007-03-01
Publication dans des actes
Résumé : In this paper, we propose an image processing method able to detect cells trough in vitro phase-contrast video microscopy. Bodies of the cells and image background are very similar; it does not allow having a good performance of tracking process. The proposed method normalizes original image to obtain better object-background contrast, image is equalized to highlight the object to be tracked, and image is threshold in order to obtain a better object-background contrast. Gray Level Morphological Gradient is applied to obtain more defined contours in the areas surrounded by Halos. Dilatation and Erosion were combined to achieve this goal. A subtraction process was applied after dilation of image to obtain borders. © Springer-Verlag Berlin Heidelberg 2007.