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Rapid up-regulation of P2Y messengers during granulocytic differentiation of HL-60 cells.

par Communi, Didier ;Janssens, Rodolphe ;Robaye, Bernard ;Zeelis, Natacha;Boeynaems, Jean-Marie
Référence FEBS letters, 475, 1, page (39-42)
Publication Publié, 2000-06

Article révisé par les pairs

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Résumé :

HL-60 cells are human promyelocytic cells expressing two ATP receptors: the P2Y(2) and P2Y(11) subtypes. Our Northern blotting experiments have shown that P2Y(2) and P2Y(11) messengers were up-regulated in these cells, rapidly and independently of protein synthesis, following treatment with granulocytic differentiating agents such as retinoic acid, dimethylsulfoxide, granulocyte-colony stimulating factor, dibutyryl cyclic AMP and ATP. AR-C67085 and adenosine 5'-O-(3-thiotriphosphate), two potent agonists of the recombinant P2Y(11) receptor, increased intracellular cAMP concentration in HL-60 cells more potently than ATP itself. These observations support the conclusion that the effect of ATP on HL-60 cell differentiation is mediated by the P2Y(11) receptor.