• Citer

The yeast H+-ATPase Pma1 promotes Rag/Gtr-dependent TORC1 activation in response to H+-coupled nutrient uptake.

par Saliba, Elie ;Evangelinos, Minoas ;Gournas, Christos ;Corrillon, Florent ;Georis, Isabelle ;André, Bruno
Référence eLife, 7
Publication Publié, 2018-03

Article révisé par les pairs

• Accès en ligne
• Détails
• Contenu
• Statistiques

Résumé :

The yeast Target of Rapamycin Complex 1 (TORC1) plays a central role in controlling growth. How amino acids and other nutrients stimulate its activity via the Rag/Gtr GTPases remains poorly understood. We here report that the signal triggering Rag/Gtr-dependent TORC1 activation upon amino-acid uptake is the coupled H+influx catalyzed by amino-acid/H+symporters. H+-dependent uptake of other nutrients, ionophore-mediated H+diffusion, and inhibition of the vacuolar V-ATPase also activate TORC1. As the increase in cytosolic H+elicited by these processes stimulates the compensating H+-export activity of the plasma membrane H+-ATPase (Pma1), we have examined whether this major ATP-consuming enzyme might be involved in TORC1 control. We find that when the endogenous Pma1 is replaced with a plant H+-ATPase, H+influx or increase fails to activate TORC1. Our results show that H+influx coupled to nutrient uptake stimulates TORC1 activity and that Pma1 is a key actor in this mechanism.