Synthesis of Secretory Component by Rat Hepatocytes in Culture

ZEVENBERGEN, J.L.; MAY, Claude; Wanson, Jean-Claude; Vaerman, Jean-Pierre

doi:doi/10.1111/j.1365-3083.1980.tb00213.x

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Synthesis of Secretory Component by Rat Hepatocytes in Culture

par ZEVENBERGEN, J.L.;MAY, Claude ;Wanson, Jean-Claude

;Vaerman, Jean-Pierre
Référence Scandinavian journal of immunology, 11, 1, page (93-97)
Publication Publié, 1980

Article révisé par les pairs

Résumé :

Normal rat hepatocytes were isolated and cultivated in vitro. Synthesis of secretory component was demonstrated by its accumulation in the culture medium, as measured by radioimmunoassay; by incorporation of 14C‐leucine in the protein specifically precipitated with anti‐secretory component antiserum; and by a positive precipitin reaction of concentrated culture medium with the same antiserum. The results explain the high levels of secretory component found in rat bile and render plausible a mechanism of hepatic IgA transfer involving secretory component as the hepatocyte membrane receptor for polymeric IgA. Copyright © 1980, Wiley Blackwell. All rights reserved

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