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Detection of rotavirus in faecal specimens with a monoclonal antibody enzyme-linked immunosorbent assay: Comparison with polyclonal antibody enzyme immuno-assays and a latex agglutination test

par Sneyers, Myriam;Thiriart, Clotilde;Buryns, C.;Lambert, Adam A.F.;Collignon, Catherine;Schwers, Anne;Coppe, Ph;Antoine, H.;Franssen, Jean-Denis M.A.G. ;Urbain, Jacques ;Karelle-Bui, Lucie;Pastoret, Paul-Pierre
Référence Comparative immunology, microbiology and infectious diseases, 12, 4, page (95-104)
Publication Publié, 1989

Article révisé par les pairs

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Résumé :

Monoclonal antibodies have been produced against the 81/36F strain of rotavirus. One of them, was chosen as diagnostic reagent: it showed high ELISA reactivity with all the bovine, human and porcine rotavirus strains tested and reacted with VP6, structural protein product known to support the common rotavirus antigen. A sandwich ELISA procedure using the chosen monoclonal as "capture and detecting" antibody was performed to detect rotavirus in faecal samples from experimentally inoculated newborn calves: it always gave a negative response with meconium and a positive response for the stool specimens which rotavirus have been isolated. This assay was compared with Enzygnost and Slidex Rota Kit tests and with a non-commercial sandwich ELISA test using polyclonal antibodies: it showed more sensitivity than the agglutination test and was as sensitive as the other two tests to detect rotavirus in routine diagnostic material. The test evaluated showed no equivocal results. © 1989.