par Van Hummelen, Paul;Nüsse, Michael;Castelain, Philippe;Kirsch-Volders, Micheline 
Référence Environmental and molecular mutagenesis, 25, 4, page (269-278)
Publication Publié, 1995
Référence Environmental and molecular mutagenesis, 25, 4, page (269-278)
Publication Publié, 1995
Article révisé par les pairs
| Résumé : | We show that for the in vitro cytochalasin‐B human lymphocyte micronucleus (MN) test, the quantification of the DNA content of MN and the difference in DNA content between the two macronuclei in the binucleate cells without MN, as measured by image analysis, gives a first estimation of the aneugenic potential of a test compound. Cultures of isolated human lymphocytes were exposed either to γ‐rays as a clastogen or to carbendazim (MBC) as an an‐eugen. The lymphocytes were stained with Feulgen stain and the MN were analyzed for DNA content with a Magiscan 2A image analyzer. The mean DNA content of MN induced by MBC were statistically higher than γ‐irradiation‐induced MN. It was demonstrated that in culture the lymphocytes, as well as the MN, are in different stages of the cell cycle, but this will not affect the discriminating power of the MN DNA content when only G1 cells are considered, or when DNA content of the MN is expressed relative to the total genome. The identification of Gl and G2 cell populations from image analysis data was performed by extrapolation of DNA content data from Gl‐ and G2‐sorted lymphocytes with a FacStar plus flow sorter. It was demonstrated that in MBC‐treated cells the DNA rearrangement between the macronuclei in binucle‐ates without MN was on the average higher than in γ‐irradiated and untreated cells, which points to aneugenic effects of MBC without the formation of MN. In contrast to DNA content measurements, the area of the MN is not a reliable measure for discriminating clastogens from aneugens. © 1995 Wiley‐Liss, Inc. Copyright © 1995 Wiley‐Liss, Inc., A Wiley Company |
