Résumé : The redox couple resazurin-resorufin exhibits electrofluorochromic properties which are investigated herein by absorption and fluorescence spectroelectrochemistry, and by electrochemically coupled fluorescence confocal laser scanning microscopy (EC-CLSM). At pH 10, the highly fluorescent resorufin dye is generated at the electrode surface by the electrochemical reduction of the poorly fluorescent resazurin. Per-forming EC-CLSM at electrode surfaces allows to monitor spatially resolved electro-chemical processes in situ and in real time. Using a small (315 µm diameter) cylin-drical electrode, a steady-state diffusion layer builds up under potentiostatic conditions at −0.45 V vs. Ag