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Contribution à l'étude de l'ultrastructure de la corticosurrénale de cobaye

par Sand, G;Fruhling, Janos ;Platten Godfroid, C.
Référence Journal of microscopy, 17, 3, page (283-298)
Publication Publié, 1973

Article révisé par les pairs

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Résumé :

The morphology of the guinea pig adrenal cortex was examined under the electron microscope in greater detail because of modifications in the method of preparing the tissue for examination, i.e., a short preliminary fixation of the whole organ in glutaraldehyde and optimal preservation during the dehydration and embedding manipulations. The zona glomerulosa is characterized by a well developed rough endoplasmic reticulum but there is a little smooth endoplasmic reticulum. Few liposomes are localized in this zone, the mean cellular volume occupied by this organelle being 3.7%. The mitochondria appear conventional, with abundant matrix and few lamellar cristae. An intermediary zone between the zona glomerulosa and fasciculata, which is present in the rat adrenal cortex, was not observed. The zona fasciculata contains many liposomes, 30 and 20% of the cell volume in the external and internal zona fasciculata respectively. The liposome contents appear homogeneous and without vacuoles. The endoplasmic reticulum is mostly smooth, composed of a network of tubes 300-700 Å in diameter. The rough and smooth endoplasmic reticulum form a single morphological unit. The mitochondria have an abundant matrix and few cristae. The cristae are lamellar and arranged in bundles and the width of the intracristal space varies between 100-400 Å. The zona reticularis is characterized by a limited number of liposomes (4.4% of the cell volume), by large regions of smooth tubular endoplasmic reticulum and by small distinct areas covered with many ribosomes and polysomes. One can also observe some grouped elements of rough endoplasmic reticulum. The mitochondria show various forms, containing lamellar or more often tubular cristae. These tubular cristae resemble those of mitochondria in cells participating in steroid synthesis. The esterified cholesterol and the triglycerides are concentrated in the liposomes.