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Isolation of Nuclear Microsatellites in the African Timber Tree Lophira alata (Ochnaceae) and Cross-Amplification in L. lanceolata

par Pineiro Portela, Rosalia ;Staquet, Adrien;Hardy, Olivier J.
Référence Applications in plant sciences, 3, 10, A3
Publication Publié, 2015-10

Article révisé par les pairs

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Résumé :

Premise of the study: Microsatellite markers were isolated in the rainforest tree Lophira alata (Ochnaceae), an important timber tree from Central Africa, and cross-amplified on its savannah counterpart, L. lanceolata. Methods and Results: From a microsatellite-enriched library sequenced on a 454 GS FLX platform, 13 primer combinations were identified. Amplification was optimized in two multiplex reactions. The primers amplified di- and trinucelotide repeats, with two to seven alleles per locus. Eleven primers also amplified in L. lanceolata. Conclusions: Microsatellite primers developed for the genus Lophira displayed sufficient variation to investigate hybridization between congeneric species in the rainforest-savannah transition.