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Development Of An Enzymatic Assay For The Determination Of Cellulose Bioavailability In Municipal Solid Waste

par Rodriguez, C.;Hiligsmann, Serge ;Ongena, Marc;Charlier, R.;Thonart, P.
Référence Biodegradation, 16, 5, page (415-422)
Publication Publié, 2005

Article révisé par les pairs

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Résumé :

[en] As there is a constant need to assess the biodegradation potential of refuse disposed ofin landfills, we have developed a method to evaluate the biodegradability of cellulosiccompounds (cellulose and hemicellulose) in municipal solid waste. This test is based onthe quantification of monosaccharids released after the hydrolysis of solid wastesamples with an optimised enzyme preparation containing commercially availablecellulases and hemicellulases. We show that the amounts of monosaccharids could berelated to the biodegradability of the cellulosic material contained in the samples. Thisenzymatic cellulose degradation test was assayed on 26 samples originating from twoBelgian landfills and collected at different depths. As results correlated well with thoseobtained with a classical biochemical methane potential assay, this new and rapid test issufficiently reliable to evaluate cellulose bioavailability in waste samples.