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Optimisation of culture conditions for biological hydrogen production by Citrobacter freundii CWBI952 in batch, sequenced-batch and semicontinuous operating mode

par Hamilton, C.;Hiligsmann, Serge ;Beckers, L.;Masset, J.;Wilmotte, A.;Thonart, P.
Référence International journal of hydrogen energy, 35, page (1089-1098)
Publication Publié, 2010

Article révisé par les pairs

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Résumé :

[en] Investigations were carried out to determine the effect of the pH, the nitrogen source, ironand the dilution rate (h 1) on fermentative hydrogen production from glucose by the newlyisolated strain Citrobacter freundii CWBI952. The hydrogen production rate (HPR), hydrogenyield, biomass and soluble metabolites were monitored at 30 C in 100 mL serum bottlesand in a 2.3 L bioreactor operated in batch, sequenced-batch and semicontinuous mode.The results indicate that hydrogen production activity, formate biosynthesis and glucoseintake rates are very sensitive to the culture pH, and that additional formate bioconversionand production of hydrogen with lower biomass yields can be obtained at pH 5.9. Ina further series of cultures casein peptone was replaced by (NH4)2SO4, a low cost alternativenitrogen source. The ammonia-based substitute was found to be suitable for H2production when a concentration of 0.045 g/L FeSO4 was provided. Optimal overallperformances (ca. an HPR of 33.2 mL H2/L h and a yield of 0:83 molH2