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A new nucleic acid-protein cross-linking reagent

par Oste, Christian ;Parfait, Raymond ;Bollen, Alex ;Crichton, Robert R.R.
Référence Molecular & general genetics : MGG, 152, 3, page (253-257)
Publication Publié, 1977-01

Article révisé par les pairs

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Résumé :

A new photoactivable reagent is described, which allows the formation of RNA-protein crosslinks via disulfide bridges in combination with mercaptobutyrimidate. The reconstituted L24 protein-23S RNA complex from the large subunit of E. coli ribosomes has been used as a model system for the cross-linking. The main advantages of the reagent are the absence of U.V. generated cross-links, since photoactivation is carried out at 360 nm, on one hand and the ease of cleavage of the cross-link by mild reduction (β-mercaptoethanol) on the other. © 1977 Springer-Verlag.