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α-Melancoyte-stimulating hormone binding and biological activity in a human melanoma cell line

par Legros, Franz Jacques ;Coel, Jean ;Doyen, Albert
Référence Cancer research, 41, 4, page (1539-1544)
Publication Publié, 1981

Article révisé par les pairs

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Résumé :

Synthetic α-melanocyte-stimulating hormone (α-MSH) was found to bind to the plasma membrane of the HM6A human melanoma cell line, using an immunocytochemical method. When treated with 10-7 to 10-9 M α-MSH, melanoma cells exhibited an increase of intracellular cyclic adenosine 3':5'-monophosphate, followed by stimulation of tyrosinase activity. Significant inhibiton of DNA synthesis measured by [3H]thymidine uptake and inhibition of cell growth was found. A retrovirus expression was detected in the supernatant of HM6A cells as assayed by the KC cell syncytium-forming test. In the presence of 10-7 M α-MSH, the number of syncytium-forming units was increased 15-fold. These results demonstrate that α-MSH modulates human melanoma differentiation and virus expression in vitro.