par Dubois, Frank ;Yourassowsky, Catherine
Référence Proceedings of SPIE - The International Society for Optical Engineering, 8429, 84291E
Publication Publié, 2012
Article révisé par les pairs
Résumé : We developed a new off-axis digital holographic microscope (DHM), working in transmission with a RGB LED illumination. This partially coherent multi-wavenlenght source gives a low noise holograms and the intensity image quality is fully exploitable for microscopy purposes. We implemented a full off-axis configuration enables the recording of the holographic data in one shot. As we used for the first tests a monochromatic CCD camera, the holograms are recorded separately in each spectral channel. The holograms are individually processed by the Fourier method to obtain, in each color, the complex amplitudes and the corresponding intensities and optical phases. The color intensities are recombined to obtain a composite RGB color image. The digital holographic refocusing and the optical phase map computation are successfully demonstrated. The multi-wavelengths operation provides a significant improvement of the collected information for colored samples. The full one-shot color hologram recording should be available with a color camera. © 2012 Copyright Society of Photo-Optical Instrumentation Engineers (SPIE).