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Efficiency of bioconversion of steroids by Fusarium oxysporum into Δ1,4-androstadiene-3,17-dione using gas chromatography

par Vincke, Bernard ;Patriarche, Gaston ;Devleeschouwer, Michel ;Crotteux, Jeanne
Référence International journal of pharmaceutics, 32, 1, page (61-69)
Publication Publié, 1986

Article révisé par les pairs

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Résumé :

Gas-liquid chromatography is suitable for the study of the bioconversion yield of dehydroisoandrosterone into Δ1,4-androstadiene-3, 17-dione. Fusarium oxysporum (a wild isolated strain) exhibits the greatest activity for this bioreaction. The optimalization of the bioconversion efficiency has been studied in culture media or in buffered solutions using free and immobilized cells. Microbiological (cell weight, choice of strains and substrate, induction) and physicochemical (pH, temperature, ionic strength and choice of buffers, organic solvents, artificial cofactors) factors influencing the reaction yield are discussed. The same operating conditions can be used for free and immobilized cells. For both techniques, about 90% of Δ1,4-androstadiene-3,17-dione is formed in 48 h with non-induced cells and in 24 h with induced cells. A simple prototype of a laboratory bioreactor is employed and tested in order to describe the advantages and limitations. © 1986.