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Amplification of exon 11 of the gene for the α-chain of β-N- acetylhexosaminidase in single human blastomeres

par Sermon, Karen;Lissens, Willy;Devroey, Paul;Van Steirteghem, André;Liebaers, Ingeborg
Référence Fertility and sterility, 63, 2, page (407-409)
Publication Publié, 1995

Article révisé par les pairs

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Résumé :

Objective: To evaluate the possibility of using polymerase chain reaction (PCR) technology for preimplantation diagnosis for Tay-Sachs disease. Design: Polymerase chain reaction on single human blastomeres. Setting: Bad quality embryos from IVF analyzed in an academic research genetics lab. Interventions: Patients underwent standard IVF procedures as infertility treatment. Results: Amplification was seen in 89.5% of the blastomeres; only one blank was contaminated. Conclusion: The technique of PCR on single blastomeres is ready to be used in clinical preimplantation diagnosis for Tay-Sachs disease.