Characterization of monoclonal antibodies against the p17 core protein of the human immunodeficiency virus 1

Cogniaux, Jacqueline; De Schepper, Nadine; Blondiau, Marie Louise; Cornet, Bernard; Horal, Peter; Vahlne, Anders

doi:doi/10.1016/0022-1759(90)90207-C

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Characterization of monoclonal antibodies against the p17 core protein of the human immunodeficiency virus 1

par Cogniaux, Jacqueline ;De Schepper, Nadine

;Blondiau, Marie Louise ;Cornet, Bernard

;Horal, Peter ;Vahlne, Anders
Référence Journal of immunological methods, 128, 2, page (165-175)
Publication Publié, 1990-04

Article révisé par les pairs

Résumé :

Five mouse hybridomas which produce monoclonal antibodies against the p17 core protein of HIV-1 have been isolated. Cross-competition assays and mapping with synthetic peptides demonstrate that two closely related epitopes are identified by these antibodies. Directed against two neighbouring peptides at the carboxy-terminal end of the molecule, they can be used for the selective detection of p17 polypeptide in a viral extract or in an infected cell lysate by a solid-phase sandwich enzyme immunoassay. © 1990.

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