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Evidence for a 5'-nucleotidase in human leukemic leukocytes

par Marique, D.;Hildebrand, Jerzy
Référence Clinica chimica acta, 45, 1, page (93-98)
Publication Publié, 1973-04

Article révisé par les pairs

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Résumé :

Fifteen preparations of leukocytes isolated from patients with chronic leukemia, 9 lymphogenic (CLL) and 6 myelogenous (CML), were tested for 5'-nucleotidase activity. The presence of 5'-nucleotidase was demonstrated in 5 cases: four CLL and one CML. The properties of this enzyme were similar to those of 5'-nucleotidase from other tissues: its activity was dependent on Mg2+, and was not inhibited by tartrate, could not be solubilized by freezing and thawing and the enzyme was active at alkaline pH. On the other hand, an enzyme active on 5'-AMP in acid medium, found in all preparations, shared several characteristics with acid phosphatase. It was inhibited by tartrate, was not affected by the absence of Mg2+ and was partially solubilized by freezing and thawing. © 1973.