par Stenuit, K.;Geuskens, Maurice 
;Steinert-Meulemans, Gilberte ;Tencer, Renée
Référence Experimental cell research, 105, 1, page (159-168)
Publication Publié, 1977-03
;Steinert-Meulemans, Gilberte ;Tencer, Renée Référence Experimental cell research, 105, 1, page (159-168)
Publication Publié, 1977-03
Article révisé par les pairs
| Résumé : | Concanavalin A (ConA) binding to Xenopus oocytes was studied by fluorescent and electron microscopy. ConA binds to the vitelline layer around the oocytes and to the vitelline envelope of mature eggs. However, when oocytes are removed from the follicles with forceps, a regular hexagonal pattern of fluorescence can be seen: the fluorescence is due to the binding of ConA to the vitelline layer and the network is generated by the remaining follicle cells which allow the lectin to reach the vitelline layer only at their junctions or extremities. ConA binding sites have also been detected on the cortical granules and at the edge or the yolk platelets. On the other hand, the plasma membrane of the oocytes does not show appreciable binding of ConA neither during oogenesis nor after maturation. No information on modifications of the membrane during oogenesis or maturation or on any anisotropy at the surface of the egg could be gathered from the study of ConA binding. © 1977. |
