par Dogusan, Zeynep 
;Book, Melissa M.L.;Verdood, Peggy;Yu-Lee, Li Yuan;Peters, Elisabeth
Référence European cytokine network, 11, 3, page (435-442)
Publication Publié, 2000
;Book, Melissa M.L.;Verdood, Peggy;Yu-Lee, Li Yuan;Peters, Elisabeth Référence European cytokine network, 11, 3, page (435-442)
Publication Publié, 2000
Article révisé par les pairs
| Résumé : | It has been proposed that prolactin (PRL) is a lympho-hemopoietic growth and differentiation factor. We show here by Western blotting that PRL-receptors (PRL-R) are expressed in normal rat bone marrow and spleen cells. We also show that PRL stimulates the phosphorylation of the PRL-R-associated Janus tyrosine kinase (JAK)-2 in rat bone marrow and spleen cells. This leads to the activation and subsequent binding of signal transducer and activator of transcription (Stat) 5b to an interferon regulatory factor-1 (IRF-1) gamma activation sequence (GAS) as visualized by electromobility shift assay. As shown after reverse transcription of mRNA by polymerase chain reaction, PRL, at physiological concentrations (0.01 μg/ml), stimulates the expression of the IRF-1 gene in these normal cells. PRL could thus affect several aspects of the immune response. |
