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Determination of selenium in human body fluids by hydride-generation atomic absorption spectrometry. Optimization of sample decomposition

par Welz, Bernhard;Melcher, Marianne;Neve, Jean
Référence Analytica chimica acta, 165, C, page (131-140)
Publication Publié, 1984

Article révisé par les pairs

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Résumé :

The accuracy of the determination of selenium in human body fluids by hydride-generation a.a.s. depends critically upon the sample decomposition-method used. Digestion with HNO3 alone gave low selenium recoveries, but with nitric, sulfuric and perchloric acids at a final temperature of 310°C gave results that agreed with those obtained by other techniques. The recovery of selenomethionine added to whole blood and of trimethylselenonium iodide added to urine was 97-104%. The average selenium values found for 6 healthy individuals were 88 μg l-1 in whole blood, 75 μg l-1 in blood plasma and 307 μg (kg Hb)-1 in erythrocytes. A detection limit of 5 μg l-1 Se in body fluids was found under routine conditions. © 1984.