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Focalisation isoélectrique de la nadh: methémoglobine réductase et des NAD(P)H : diaphorases de l'érythrocyte humain

par Sonnet, Jean;de Noyette, J.P.;Steichen, Marc
Référence Clinica chimica acta, 32, 1, page (5-16)
Publication Publié, 1971

Article révisé par les pairs

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Résumé :

With this technique, the hemoglobin and enzymes of a human red cell hemolysate migrate through a pH gradient of 4 to 9, obtained with carrier ampholytes, within a thin layer of polyacrylamide gel. The red cell NAD(P)H : oxidoreductases are fractionated into three components, while the bulk of hemoglobin is resolved into four or five discrete bands. The procedure is particularly promising for the study of the NADH: methemoglobin oxidoreductase or its molecular variants and allows the use of crude red cell hemolysates. © 1971.