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A simple and rapid solid-phase radioimmunoassay for serum progesterone, using the protein A of Staphylococcus aureus as immunoadsorbent

par Jungers, J.;Desnoeck, Josiane Bertha ;Robyn, Claude
Référence The Journal of Laboratory and Clinical Medicine, 98, 1, page (30-36)
Publication Publié, 1981

Article révisé par les pairs

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Résumé :

A simple, rapid, and inexpensive radioimmunoassay method for serum progesterone is described, which uses a solid-phase technique for separation of antibody-bound from antibody-free progesterone. Rabbit antiprogesterone immunoglobulins are adsorbed on the protein A of formaldehyde- and heat-treated Staphylococcus aureus cells (Pansorbin; Calbiochem-Behring Corp., La Jolla, Calif.). The suspension of antibody-coated Pansorbin retains all its binding activity (23% for 500 μl of a 0.033% suspension) of 1-2-H(N)-progesterone (20,000 cpm in 100 μl) when kept at +4 ° or at -25 ° C for at least 4 months. Dose-response curves obtained with ether-serum extracts and with the progesterone standard do not deviate significantly from parallelism. The progesterone standard gives identical dose-response curves whether diluted in the assay buffer or in a progesterone-free ether-serum extract. The sensitivity of the assay is 0.02 ng/assay tube. The intra-assay variation coefficient is 16%, and the routine interassay variation coefficient is 17%. The mean serum progesterone concentrations were 0.55 ng/ml during the follicular phase of the menstrual cycle and 12.5 ng/ml during the luteal phase. The average blank value for distilled water was 0.02 ng/assay tube. © 1981.