par Dacremont, Georges;Hildebrand, Jerzy 
Référence Biochimica et biophysica acta. L, Lipids and lipid metabolism, 424, 2, page (315-322)
Publication Publié, 1976-02
Référence Biochimica et biophysica acta. L, Lipids and lipid metabolism, 424, 2, page (315-322)
Publication Publié, 1976-02
Article révisé par les pairs
| Résumé : | Eight bands of gangliosides, from human polymorphonuclear leukocytes were demonstrated by thin-layer chromatography. Bands 4 and 5 were isolated and purified in sufficient amounts to allow their biochemical identification by thin-layer chromatography, gas chromatography and sequential action of glycosidases and neuraminidase. The major ganglioside was characterised as N-acetylneuraminylgalactosyl-β-N-acetylglucosaminyl-β-galactos yl-β-glucosylceramide. A second ganglioside was tentatively identified as N-acetylneuraminyl-galactosyl-β-N-acetylglucosaminyl-β-(N-acet ylneuraminyl) galactosyl-β-glucosyleeramide. Both gangliosides isolated were hydrolysed by neuraminidase. However, treatment of the intact cells with neuraminidase did not alter the ganglioside pattern. © 1976. |
