par Shugar, David 
Référence Biochimica et biophysica acta, 6, C, page (548-561)
Publication Publié, 1950
Référence Biochimica et biophysica acta, 6, C, page (548-561)
Publication Publié, 1950
Article révisé par les pairs
| Résumé : | 1. 1. The inactivation of triosephosphate dehydrogenase by ultra-violet light of wave-length 2537 Å is first-order. The quantum yield at this wave-length and at the isoelectric point is 3.2·10-3. 2. 2. The quantum yield appears to depend upon the concentration of the irradiated solution. 3. 3. In the presence of the reducing agent, cysteine, partial protection against inactivation is obtained; but the inactivation rate is no longer 1st order. 4. 4. The rate of inactivation is not affected by prior reduction of the enzyme-bound coenzyme DPN. 5. 5. Following reduction of the bound DPN, irradiation in the near ultraviolet results in a partial increase in enzyme activity. 6. 6. The nature of this photoactivation process, as well as the other observations, is discussed. © 1950. |
