Dibekacin assay in serum by automated fluorescence polarization immunoassay (Abbott Tdx): Comparison with high-performance liquid chromatography, substrate-labelled fluorescent immunoassay and radioimmunoassay

Rollman, Bruno; Tulkens, Paul M.; Van Der Auwera, Philippe

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Dibekacin assay in serum by automated fluorescence polarization immunoassay (Abbott Tdx): Comparison with high-performance liquid chromatography, substrate-labelled fluorescent immunoassay and radioimmunoassay

par Rollman, Bruno ;Tulkens, Paul M.;Van Der Auwera, Philippe

Référence Journal of pharmaceutical and biomedical analysis, 4, 1, page (53-61)
Publication Publié, 1986

Article révisé par les pairs

Résumé :

An immunoassay based on fluorescence polarization detection (FPIA) has been recently adapted for dibekacin. This has been compared with a reference method (high-performance liquid chromatography), and two other methods used in clinical laboratories for monitoring this aminoglycoside, namely substrate-labelled fluorescent immunoassay (SLFIA) and radioimmunoassay (RIA). FPIA was fast and more reliable than SLFIA or RIA, and offered therefore superior performance. However, its nominal cost per assay is high. © 1986.

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