par Steinert, Maurice 
;Van Assel, Suzanne
Référence Plasmid, 3, 1, page (7-17)
Publication Publié, 1980
;Van Assel, Suzanne Référence Plasmid, 3, 1, page (7-17)
Publication Publié, 1980
Article révisé par les pairs
| Résumé : | Melting and reannealing of purified kinetoplast DNA (kDNA) from Crithidia fasciculata, Trypanosoma mega, and T. brucei have been studied with an automated optical system. The slow reassociation rate of trypanosome kDNA is due neither to the formation of hyperpolymers nor to mispairing of bases and certainly reflects extensive sequence heterogeneity. Simulation of the reassociation kinetics indicates that the kDNA comprises essentially two kinetic components: a fast renaturing component which might be a common sequence present in all the minicircles and a slow renaturing component which is responsible for minicircle heterogeneity. The rapidly renaturing component is more abundant in Crithidia than in trypanosomes. © 1980 Academic Press, Inc. All rights of reproduction in any form reserved. |
