par Masson, Pierre ;Heremans, Joseph;Prignot, Jacques
Référence Biochimica et biophysica acta (G). General subjects, 111, 2, page (466-478)
Publication Publié, 1965-12
Article révisé par les pairs
Résumé : Four samples of normal human bronchial secretions, collected without admixture of saliva, and a pool of sputum obtained from 10 patients with non-purulent chronic bronchitis, were analyzed by electrophoretic and immuno-electrophoretic methods. The sputum was, in addition, submitted to chromatographic fractionation on DEAE-cellulose and the fractions thus obtained were further characterized by electrophoresis, immuno-electrophoresis and tested for the presence of amylase and lysozyme. Albumin and γA-immunoglobulin were the main plasma proteins identified in all samples, whereas γG-immunoglobulin was present only in negligible amounts. The proteins specific for saliva were identified in the sputum samples, but four of them, viz. lysozyme, lactoferrin, salivary β1-globulin and (probably) salivary α2-globulin, were also identified in bronchial samples uncontaminated by saliva. © 1965.