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In situ fluorescence microscopy study of the interfacial inhomogeneity of DNA mixed self-assembled monolayers at gold electrodes

par Meunier, Anne ;Triffaux, Eleonore ;Bizzotto, D.;Buess Herman, Claudine ;Doneux, Thomas
Référence ChemElectroChem (Weinheim), 2, 3, page (434-442)
Publication Publié, 2015

Article révisé par les pairs

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Résumé :

Mixed self-assembled monolayers (SAMs) composed of a fluorescently labeled DNA and a mercaptobutanol diluent immobilized on gold electrodes were characterized by electrochemical measurements coupled with in situ fluorescence microscopy. The reductive desorption of the SAMs was monitored in real time through the variations of capacitance and fluorescence intensity. Desorption occurs in several steps, related to the substrate crystallinity. Fluorescence microscopy revealed the presence of spatial heterogeneities in the form of highly fluorescent aggregates that remain at the electrode surface even after a reductive desorption step.This in situ electrofluorescence microscopy technique is useful to optimize the formation of the mixed layer to obtain a homogeneous distribution of the probes and thus to improve the efficiency of the recognition process in the development of biosensors.