par Geuskens, Maurice ;de Jonghe d'Ardoye, V.
Référence Experimental cell research, 67, 1, page (61-72)
Publication Publié, 1971-07
Article révisé par les pairs
Résumé : Polar lobes detached from Ilyanassa embryos at the trefoil stage were incubated in the presence of radioactive uridine or thymidine for variable periods and studied by autoradiography. In vitro, an hyaline region devoid of yolk platelets is formed inside the lobes. In this region the incorporation of 3H-uridine is intense, that of 3H-thymidine is only slight. A high proportion of the radioactivity resulting from the incorporation of labelled uridine is resistant to both ribonuclease and deoxyribonuclease. An ultrastructural study has shown that this precursor is mainly incorporated into mitochondria, the peripheral zone of the yolk platelets, polyribosomes, and multimembranous vesicles. The activity localized in the mitochondria, the yolk platelets and the multimembranous vesicles is resistant to ribonuclease. The nature of the labelled macromolecules and the contribution of our observations to the understanding of the morphogenetic influence exerted by the polar lobe are discussed. © 1971.