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The rôle of the nucleolus in ribonucleic acid- and protein synthesis. II. Amino acid incorporation into normal and nucleolar inactivated hela cells

par Errera, Maurice Leo ;Hell, A.;Perry, Robert R.P.
Référence Biochimica et biophysica acta, 49, 1, page (58-63)
Publication Publié, 1961-04

Article révisé par les pairs

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Résumé :

The kinetics of incorporation of amino acids into the different parts of growing HeLa cells indicate that the process begins at a comparable rate in the nucleolus, in the rest of the nucleus, and in the cytoplasm. Therefore there seems in our circumstances to be no direct relationship between nucleoside uptake and amino acid uptake. If the nucleolus is irradiated with a u.v. microbeam, with a dose such as to inhibit almost completely cytidine incorporation, a partial effect (30% inhibition) on amino acid uptake in this organelle only becomes apparent after a period of incubation of 6 h. In these conditions there is no change in amino acid incorporation into the rest of the nucleus, but there is a 30% drop in amino acid incorporation into the cytoplasm, indicating some remote control of the nucleolus on cytoplasmic protein metabolism. © 1961.