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The effects of γ irradiation on the hydration characteristics of DNA and polynucleotides. I. An NMR study of frozen H2O and D2O solutions

par Mathur De Vre, R.;Bertinchamps, Albert ;Berendsen, Herman Jc C
Référence Radiation research, 68, 2, page (197-214)
Publication Publié, 1976

Article révisé par les pairs

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Résumé :

Water proton and deuteron resonance spectra have been studied in frozen solutions of DNA, poly(A), poly(C), poly(U), poly(A+U) and poly (A+2U) at different temperatures between -5 and -40°C. Hydration water molecules exhibit high proton and molecular mobility in frozen solutions and are responsible for the observed spectra. The γ irradiation of solutions at O and -80°C results in striking changes in the proton spectra and no change in the deuteron spectra. These results suggest that proton exchange is an important factor in affecting the proton linewidth and the proton relaxation mechanism. The changes in molecular structure (either by γ irradiation or molecular association) influence the hydration water in a characteristic manner. The effects of irradiation at different temperatures (O, -80, and -196°C) suggest that hydration water makes a distinct contribution to the overall radical attack on the DNA molecule from the water medium.