• Citer

Complementary action of recombination and excision in the repair of ultraviolet irradiation damage to DNA

par Radman, Miroslaw ;Cordone, Lorenzo;Simic, Draga ;Errera, Maurice Leo
Référence Journal of Molecular Biology, 49, 1, page (203-212)
Publication Publié, 1970-04

Article révisé par les pairs

• Détails
• Contenu

Résumé :

The roles of the bacterial rec and uvr and of the λ red functions for the survival of ultraviolet-irradiated λ phage have been studied. If only a small number of pyrimidine dimers is present in the λ DNA, repair is preferentially performed by recombination (rec and red functions); excision repair (uvr functions) being efficient only for extensive damage. This is probably related to the ultraviolet-induced inhibition of DNA replication. A temporary inhibition of post-irradiation DNA replication was obtained using a λ, cistron O, thermosensitive mutant. It was thereby demonstrated that excision repair is efficient on non-replicating and recombination repair on replicating DNA. It is suggested that recombination and excision are complementary mechanisms for repairing ultraviolet irradiation damage to DNA, excision taking place before DNA replication and recombination repair after and/or during replication. Regulation of DNA replication is therefore an important factor for the efficient repair of DNA. © 1970.