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Detection of H-Y in the enzyme-linked immunosorbent assay

par Farber, Claire ;Liebenthal, Daniel;Wachtel, Stephen S.S.;Cunningham-Rundles, Charlotte
Référence Human genetics, 65, 3, page (278-279)
Publication Publié, 1984-01

Article révisé par les pairs

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Résumé :

We have developed a new enzyme-linked immunosorbent assay for determination of H-Y phenotype in the human. This assay, which measures the inhibition of the reaction of a monoclonal anti-H-Y antibody and a mouse testis extract as a source of H-Y antigen, was applied to the supernatant of lymphocytes from ten normal male and ten normal female subjects. Introduction of supernatant from male cells gave reading of 69%-78% of those obtained with testis supernatant alone; female-cell supernatant did not inhibit the reaction (89%-102%). © 1984 Springer-Verlag.