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Identification of the Amino Acid Residues Affecting the Catalytic Pocket of the Sulfolobus solfataricus Signature Amidase

par Cilia, Elisa ;Ammendola, Sergio
Référence Protein peptide letters, 17, 2, page (146-150), 5
Publication Publié, 2010-02-01

Article révisé par les pairs

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Résumé :

36 mutants of the Sulfolobus solfataricus amidase were analyzed by comparing biochemical data to structural data obtained by a learning machine. The analysis shows that beside well known catalytic residues, amino acid residues Arg197, Lys209 and Asp228 are important for the catalytic activity of the signature thermophilic amidase.