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The rosetting factor of human mixed lymphocyte cultures

par Wybran, Joseph ;Govaerts, André
Référence Clinical immunology and immunopathology, 9, 2, page (240-247)
Publication Publié, 1978

Article révisé par les pairs

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Résumé :

The aim of this study was to investigate a possible action of human mixed lymphocyte culture (MLC) supernatants on rosette formation between T lymphocytes and sheep red blood cells. The supernatants of MLC between unrelated donors A and B were compared to the supernatants of A or B lymphocytes cultured alone for periods between 1 hr and 4 days. Lymphocytes of a third unrelated donor C were incubated at 37°C with the various supernatants, and rosette formation was measured by both the active T-rosette assay and the total T-rosette assay. It was found that supernatants of MLC increased the percentage of active rosettes (TEa) compared to A or B supernatants. There was no increase in the total T rosettes (TEt). The effect was detectable when lymphocytes were cultured for only 1 hr. At Day 4, the TEa were 24.1 ± 7.6% for A supernatants, 24.2 ± 7.1% for B supernatants, and 36.8 ± 8.5% for AB supernatants (MLC). The TEt were, respectively, 66.6 ± 4.0, 65.0 ± 4.4, and 66.0 ± 1.8%. Cycloheximide and mitomycin treatment of A and B lymphocytes abolished the rosetting property of MLC supernatants. Heat also destroyed this property. It is concluded that MLC supernatants contain a factor termed the rosetting factor (RF), actively synthesized by lmyphocytes, which is capable of acting on a subpopulation of T lymphocytes. This factor may be of importance in immune competence in view of the known correlation of TEa with cellular immunity.