par Van den Bergh, Karolien;Vercammen, Martine J.;Regenass, Stephan;Derua, Rita;Vermeersch, Pieter;Pokreisz, Peter;Ocmant, Annick 
;de Beéck, Katrijn Op;Janssens, Stefan;Waelkens, Etienne;Bossuyt, Xavier
Référence Clinica chimica acta, 413, 1-2, page (105-108)
Publication Publié, 2012-01
;de Beéck, Katrijn Op;Janssens, Stefan;Waelkens, Etienne;Bossuyt, XavierRéférence Clinica chimica acta, 413, 1-2, page (105-108)
Publication Publié, 2012-01
Article révisé par les pairs
| Résumé : | Anti-Golgi antibodies are rare autoantibodies that have been described in systemic autoimmune diseases. Not all Golgi auto-antigens are known. The objective of this study was to identify a novel auto-antigen associated with anti-Golgi immune reactivity. Sera from a patient with Golgi immune reactivity and from a control individual were used for Western blotting after 2-dimensional gel separation of a rat Golgi-enriched extract.Betaine homocysteine S-methyltransferase 1 (BHMT1) was identified as an auto-antigen by MALDI-TOF/TOF mass spectrometry. Using human recombinant BHMT1, a strong positive blotting signal was obtained with serum from the patient but not from a control. Pre-absorption of the serum sample with reactivity to BHMT1 with recombinant human BHMT1 resulted in decreased reactivity on Western blotting and in disappearance of the Golgi-like pattern on indirect immunofluorescence. Using immunocytochemistry, we confirmed the subcellular localization of BHMT1 to the Golgi apparatus. Antibodies to BHMT1 were found in four of 80 samples with a Golgi-pattern on indirect immunofluorescence. The antibodies were not associated with a specific clinical condition.We identified BHMT1 as a novel auto-antigen associated with anti-Golgi immune reactivity. © 2011 Elsevier B.V. |
