par Pasquier, Christophe;Andreutti, Corinne;Bertrand, Evelyne ;Bostan, Alionka ;Bourlet, Thomas;Molina, Irene;Grossman, Zehava;Halfon, Philippe;Leruez-Ville, Marianne;Lüneborg-Nielsen, Margrethe;Mar, Carmen;Marcelin, Anne-Geneviève;Roussel-Ronserail, Catherine;Schmitt, Marie-Paule;Tabrizi, Sepehr;Vourliotis, Maria;Bujan, Louis
Référence Journal of medical virology, 84, 2, page (183-187)
Publication Publié, 2012-02
Référence Journal of medical virology, 84, 2, page (183-187)
Publication Publié, 2012-02
Article révisé par les pairs
Résumé : | Detection of HIV-1 RNA in semen is used commonly to determine the safety of semen processing procedures before assisted reproductive technology (ART). Using two panels of prepared semen samples containing HIV-1 the performances of protocols from 14 centers have been compared. No false-positive results were detected but false-negative results were frequent when the concentration was below 500 HIV-1 RNA copies/ml of seminal plasma. Frequency of HIV-1 RNA detection was higher on seminal cells than on seminal plasma. Assays (or protocols) for quantifying HIV-1 RNA in semen performed less well than standardized blood plasma assays. The HIV load in seminal plasma could be a useful marker of the risk of sexual transmission of the virus. Its use as a marker of global HAART efficiency in the HIV reservoir needs further study. Standardized assays are required for detection and measurement of HIV-1 RNA in semen samples. © 2011 Wiley Periodicals, Inc. |