Experimental evolution of enzyme temperature activity profile: Selection in vivo and characterization of low-temperature-adapted mutants of Pyrococcus furiosus ornithine carbamoyltransferase

Roovers, Martine; Sanchez, Rony; Legrain, Christiane; Glansdorff, Nicolas

doi:doi/10.1128/JB.183.3.1101-1105.2001

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Experimental evolution of enzyme temperature activity profile: Selection in vivo and characterization of low-temperature-adapted mutants of Pyrococcus furiosus ornithine carbamoyltransferase

par Roovers, Martine ;Sanchez, Rony ;Legrain, Christiane

;Glansdorff, Nicolas

Référence Journal of bacteriology, 183, 3, page (1101-1105)
Publication Publié, 2001

Article révisé par les pairs

Résumé :

We have obtained mutants of Pyrococcus furiosus ornithine carbamoyltransferase active at low temperatures by selecting for complementafion of an appropriate yeast mutant after in vivo mutagenesis. The mutants were double ones, still complementing at 15°C, a temperature already in the psychrophilic range. Their kinetic analysis is reported.

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