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Temperature dependence of delayed ehlorophyll fluorescence in intact leaves of higher plants. A rapid method for detecting the phase transition of thylakoid membrane lipids

par Havaux, Michel ;Lannoye, Robert
Référence Photosynthesis research, 4, 1, page (257-263)
Publication Publié, 1983-01

Article révisé par les pairs

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Résumé :

The temperature dependence of the yield of in vivo prompt and delayed chiorophyll fluorescence was investigated in maize and barley leaves. In the chilling-sensitive maize, delayed fluorescence at steady-state level showed a maximum near the temperature at which thylakoid membrane lipids undergo a phase transition as revealed by differential scanning calorimetry measurements. In the chilling-resistant barley, no phase transition was detected above 0°C and the delayed light emission varied in a monotonic fashion. It was shown that measurements of delayed luminescence intensity in vivo can provide a rapid and sensitive method for detecting the phase change of membrane lipids in intact leaves of chilling-sensitive plant species such as tomato, cotton, cucumber, castor bean or avocado. In contrast, the use of steady-state prompt chlorophyll fluorescence as an indicator of membrane fluidity change was not successful. © 1983 Martinus Nijhoff/Dr W. Junk Publishers.