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In vitro response of subpopulations of human lymphocytes. II. DNA synthesis induced by anti immunoglobulin antibodies

par Gausset, Philippe ;Delespesse, Guy ;Hubert, Claude
Référence The Journal of immunology, 116, 2, page (446-453)
Publication Publié, 1976

Article révisé par les pairs

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Résumé :

A significant and constant increase in DNA synthesis was observed in human lymphocytes cultered in the presence of purified anti immunoglobulin antibodies specific for human IgG, IgA and IgM. This was found in cultures of lymphocytes isolated from blood, tonsils, spleen, and lymph nodes. The optimal culture conditions for blood and tonsil lymphocytes were determined. As a rule 6 day cultures containing 2 x 10 6 cells/ml and 100 μg/ml of antibody yielded the highest 3H thymidine uptake. Purified T cell cultures could not be stimulated, whereas a low response could be observed in most of the purified B cell cultures. Optimal culture conditions were the same for the B and total tonsil lymphocytes. However, when the purified B cells were totally depleted of T cells, no response was observed. A T and B cell synergy has been demonstrated by supplementing B cell cultures with purified T cells, whether treated or not with mitomycin. These experiments indicated a permissive and potentiating effect of T cells on the B cell response. Cultures containing mitomycin treated B cells and purified T cells (mB + T) could be stimulated by a Ig, thus indicating a T cell proliferation. In keeping with this finding was the observation of an increased response of total lymphocytes supplemented with T cells but not with B cells. Adherent cells are necessary for an optimal response to a Ig; they enhanced the B cell proliferation observed in (Tm + B) cultures and suppressed the response of T cells in (T + Bm) cultures.