par Lane, Charles;Marbaix, Gérard ;Gurdon, John
Référence Journal of Molecular Biology, 61, 1, page (73-91)
Publication Publié, 1971-10
Article révisé par les pairs
Résumé : 4 s + 5 s RNA, 9 s RNA, 18 s RNA, 28 s RNA and polyribosomes, all prepared from rabbit reticulocytes, were injected into living oocytes of the frog Xenopus laevis. Only after injection of 9 s RNA or polyribosomes was haemoglobin-like material formed. Haemoglobin was identified by various criteria: gel filtration, carboxymethyl cellulose chromatography, acrylamide gel electrophoresis and ion exchange chromatography of tryptic peptides. 9 s RNA promoted the formation of globin-like material, whether injected with haemin or without haemin. It is concluded that when injected into a living cell, the 9 s RNA is fairly stable and has the properties of a haemoglobin messenger. The messenger requires no reticulocyte-specific factors for translation, and the translational machinery of the oocyte will accept the messenger RNA from a totally different cell type, from another species. At all stages of assembly and synthesis haemoglobin molecules are reasonably stable in oocytes. © 1971.