par Mori, Marcella;Benotmane, Mohammed Abderrafi;Tirone, I.;Peters, Elisabeth 
;Desaintes, Christian
Référence Cellular and molecular life sciences, 62, 13, page (1489-1501)
Publication Publié, 2005-07
;Desaintes, Christian Référence Cellular and molecular life sciences, 62, 13, page (1489-1501)
Publication Publié, 2005-07
Article révisé par les pairs
| Résumé : | Human lymphocyte subpopulations differ in their cellular responses to ionizing radiation. To shed light on the molecular basis of this effect, we characterized the transcriptional response to 1 Gy X-rays of CD4+ T lymphocytes. Of 18,433 genes tested, 102 were modulated more than 1.5-fold. The majority of the strongly activated genes were p53 targets involved in DNA repair and apoptosis. The expression of three of these genes was further tested by quantitative RT-PCR in lymphocyte subpopulations [CD4+ and CD8+ T, CD19+ B, CD56+ natural killer cells and peripheral blood lymphocytes (PBLs)] from ten adult donors. In contrast to DDB2, TNFRSF10B and BAX were differentially modulated among the subpopulations and the PBLs, being more activated in irradiated CD19+ B and CD8+ T lymphocytes. The level of BAX activation in the various subpopulations correlated with the sensitivity of the cells to radiation, suggesting its possible role in the differential radiosensitivity of hematopoietic cell subsets. © Birkhäuser Verlag, 2005. |
