Article révisé par les pairs
Résumé : We developed a Digital Holographic Microscope (DHM) workingwith a partial coherent source specifically adapted to perform highthroughput recording of holograms of plankton organisms in-flow, in a sizerange of 3μm-300μm, which is of importance for this kind of applications.This wide size range is achieved with the same flow cell and with the samemicroscope magnification. The DHM configuration combines a highmagnification with a large field of view and provides high-resolutionintensity and quantitative phase images refocusing on high sample flowrate. Specific algorithms were developed to detect and extract automaticallythe particles and organisms present in the samples in order to buildholograms of each one that are used for holographic refocusing andquantitative phase contrast imaging. Experimental results are shown anddiscussed.