Résumé : Context - During the last two decades, functional biodiversity research has provided strong support for the hypothesis that more biodiverse ecosystems have the potential to deliver more and better services. However, most empirical support for this hypothesis comes from simple structured communities that are relatively easy to manipulate. The impact of forest biodiversity on forest ecosystem functioning has been far less studied. Experiment design - In this paper, we present the recently established, large-scale FORBIO experiment (FORest BIOdiversity and Ecosystem Functioning), specifically designed to test the effects of tree species diversity on forest ecosystem functioning. FORBIO's design matches with that of the few other tree diversity experiments worldwide, but at the same time, the FORBIO experiment is unique as it consists of a similar experimental set-up at three sites in Belgium (Zedelgem, Hechtel-Eksel and Gedinne) with contrasting edaphic and climatological characteristics. This design will help to provide answers to one of the most interesting unresolved questions in functional biodiversity research, notably whether the effects of complementarity on ecosystem functioning decrease in less stressful and more productive environments. At each site, FORBIO consists of 41 to 44 plots (127 plots in total) planted with monocultures and mixtures up to four species, selected from a pool of five site-adapted, functionally different tree species. When allocating the treatments to the plots, we maximally avoided any possible covariation between environmental factors. Monitoring of ecosystem functioning already started at the Zedelgem and Gedinne sites and will start soon in Hechtel-Eksel. Multiple processes are being measured and as the trees grow older, we plan to add even more processes. Expected results - Not only basic science, but also forest management will benefit from the results coming from the FORBIO experiment, as FORBIO is, for instance, also a test case for uncommon, not well-known tree species mixtures. To conclude, FORBIO is an important ecosystem experiment that has the potential to deliver badly needed insights into the multiple relationships between biodiversity and ecosystem functioning, which will be valuable for both science and practice. © 2013 National Botanic Garden of Belgium and Royal Botanical Society of Belgium.